Sedum middendorffianum Maxim Induces Apoptosis and Inhibits the Invasion of Human Ovarian Cancer Cells via Oxidative Stress Regulation

Sedum middendorffianum Maxim (SMM) is a Korean endemic plant belonging to the Crassulaceae family. This study aimed investigate antitumor effects of SMM extract on human ovarian cancer cells. Among five plants grown in Korea, showed most potent cytotoxicity cells and had little effect normal surface epithelial Furthermore, we revealed that dose-dependently induced apoptosis A2780 SKOV3 The markedly stimulated activation caspase-3/8, while broad-spectrum caspase inhibitor caspase-8 selective significantly reversed extract-induced apoptosis. In addition, inhibited cell invasion expression levels matrix metalloproteinase (MMP)-2 MMP-9 Notably, increased generation intracellular ROS, pretreatment with antioxidant N-acetyl-L-cysteine (NAC) suppressed SMM-induced anti-invasive activity. Moreover, NAC treatment inhibition MMP-2/9 expression. Taken together, these data suggest induces caspase-dependent apoptotic death inhibits MMP-dependent via ROS regulation.